As part of GrasPods Welcome Week 2021, we’re delighted to bring you Part 1 of a step-by-step RNA-seq data analysis workshop, in association with the BC Children’s Hospital Research Institute’s Trainee Omics Group (TOG). TOG is the resident graduate trainee-led data bioinformatics and data analysis support group at BCCHR, offering workshops, tutorials, and seminars in biomedical statistical analyses and methodologies.

Part 1 - RNA-seq: From Raw to Processed Data
Date and Time: 7 October 2021 (Thursday) from 12 pm (noon) – 2p.m.
Requirements: Basic working-knowledge and experience with R.

Part 1 of the workshop will introduce what RNA-seq data looks like and how to clean the data. Starting with a raw expression count matrix (pre-formatted for participants’ convenience), we will go through steps to visualize, filter, and normalize the data. At the end of Part 1, our processed data will be ready to perform differential gene analysis and gene-pathway enrichment on.

Note: While this workshop will not focus on the upstream quality control of FASTQ files and genome alignment, we will briefly go through how these steps are performed. The reason for not including the above mentioned processes in the workshop is due to the need of a Linux computing system required for alignment, which some of the audience might not have immediate access to.

There’s a few pre-workshop to-dos to complete before the day of the workshop – find them here! We highly recommending completing these, as you’ll be needing a few files and R packages already downloaded and installed, which we will be using during the workshop.